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Superoxide reporters of the DHE family yield non-specific raw fluorescence, thus quantification must rely on chromatographic separation of DHE→2-hydroxyethidium (2-OH-E⁺) by HPLC to measure O 2· − and discriminate it from ethidium or other adducts, ideally alongside SOD controls and pathway-selective perturbations (e.g., NOX2 inhibitors, mitochondrial site inhibitors) for source assignment

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Tier:Tier 3

𝗥𝗲𝗺𝗶𝗻𝗱𝗲𝗿: FY26 Release 3 topics close at 12 p.m