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Co-delivery of Interleukin-12 and doxorubicin loaded Nano-delivery system for enhanced immunotherapy with polarization toward M1-type macrophages

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Splenocytes from λ-MYC mice and wild-type littermates were isolated as previously described [53] and stained with APC-conjugated rat anti-mouse CD45R/B220 (ref 561880) and PE-conjugated rat anti-mouse CD43 (ref 561857) antibodies, both from BD Biosciences, before flow cytometry analysis with a FACS Canto II flow cytometer (BD Biosciences) employing Kaluza software (Beckman Coulter)

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