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2.4 Investigation of reactive oxygen species generation and glutathione depletion 2.4.1 Reactive oxygen species generation from Mn 2+ -mediated Fenton-like reaction MB (10 μg/ml), H 2 O 2 (1 mM), Mn 2+ (0.5 mM) and HCO 3 − (6.25, 12.5, 25, 50, or 100 mM) were incubated in 1 ml of aqueous solutions at room temperature for 30 min

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10.1016/j.jep.2009.02.015 5 Ben SlimaA.Ben AliM.BarkallahM.TraoreA