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Characterization methods for third-generation liposomes include the evaluation of the targeting ligand conjugation efficiency, receptor-mediated binding, internalization, scanning electron microscopy (SEM), and pharmacokinetic and biodistribution properties of the liposomal formulation [182]

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In our research, we found that incubation with 10 nM of CuSO 4 triggered the highest irregularity of heartbeat, which is shown by the higher sd1 value when compared with its control counterpart (Figure 5A)