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Briefly, cells were suspended in sucrose-mannitol buffer (20 mM HEPES, pH 7.5, 2 mM EDTA, 70 mM sucrose, 220 mM mannitol, 5 mM NaF, protease inhibitor cocktail [Roche]) and homogenized using a Teflon homogenizer

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7 , ra94–ra94 (2014)