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This sample was then applied to a Superdex 200 26/60 gel filtration column (GE Healthcare) and pure fractions corresponding to a molecular weight of ~82 kDa were collected and concentrated

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[Effect of hCG on the gonads of Rana esculenta in organotypic culture, during… [PMID: 5316985] Laboratory literature summary: [Effect of hCG on the gonads of Rana esculenta in organotypic culture, during metamorphosis]