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For example, in vitro experiments indicated that the HDAC inhibitor, Trichostatin A (TSA), has a significant effect on the endogenous expression of RANKL (well known for its significance in bone metabolism) through enhanced acetylation of histones H3 and H4 on the proximal RANKL promoter sequence in bone stromal cells (Fan X, et al., J Cell Biochem., 2004

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From day 1 to 3, cells were cultured in StemPro34 SFM (Invitrogen) with 50 μg ml −1 ascorbic acid (AA) (Sigma), 2 mM Gluta-MAX (Invitrogen), 10 ng ml −1 BMP4, and 10 ng ml −1 human recombinant activin-A (Invitrogen)