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122 showed the difference between the pharmacokinetic profile in preterm babies and adults caused by immature liver and poor renal excretion and concluded that a 2-h infusion of 0.1 μg/kg/h increased plasma melatonin from undetectable to approximately peak adult concentration

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The concentration and integrity of RNA samples were assessed using a Nanodrop spectrophotometer (Thermo Scientific) and agarose gel electrophoresis