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Cysteine supplementation exhibited a significant positive effect on the survival of transgenic flies ( D = 1.16, 1.09–1.25, FDR = 0.0002, n = 9) and high doses significantly reduced survival time ( D = 0.93, 0.87–0.99, FDR = 0.0396, n = 53)

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As random effects, we had intercepts for plateID (replicates), as well as by-plateID random slopes for the effect of time