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2.1.5 Neutrophil extravasation and migration experiments After 2 days of culture, 0, 10, 100 ng/mL IL-6 (130-093-929, Miltenyi Biotec) was added to the lumens and incubated at 37 ˚C and 5.0% CO 2 for approximately 45 minutes

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In humans, the importance of ascorbate as a cofactor for PAM was mentioned in connection to the beneficial effects of vitamin C administration and sepsis outcome