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SLC25A39 depletion inhibits subcutaneous xenograft growth in vivo To evaluate the effect of SLC25A39 on LUAD tumor growth in vivo, we established a xenograft model by subcutaneously injecting 2 × 10 6 A549 cells—either Cas9 control (Cas9C) or SLC25A39 knockout (koSLC25A39)—into the left axilla of nude mice (n = 7 per group)