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Determination of mitochondrial membrane depolarisation in 3T3-L1 adipocytes Changes in mitochondrial membrane potential ( ΔΨm ) were assessed by using the JC-1 fluorescent stain, according to a previously described method [16]

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The reaction is catalyzed by pyridoxal 5’-phosphate (PLP)-dependent L-seryl-tRNASec selenium transferase and uses selenophosphate (monoselenium phosphate) as the selenium donor ( Figure 4 ) (26)