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EMSA EMSAs were performed by mixing increasing amounts of wt or mutated BRD4 ET (with or without ASXL1 peptide at a 1:1 molar ratio) with 10 nM/lane of 147 bp 601 DNA in 20–25 mM Tris pH 7.5, 150 mM NaCl and 5 mM DTT in a 10 μL reaction volume