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These findings suggest that Gln metabolism should be understood more appropriately as a “metabolic network node,” whose effects depend on nutrient availability, mitochondrial functional status, and its mode of coupling with other metabolic pathways, such as glycolysis and TCA cycle interruption and anaplerosis

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The cell lysates were incubated for 4 h with 1 μg of the appropriate antibodies in the presence of Protein A/G agarose beads (InvivoGen) at 4 °C