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Subcellular fractionation and immunoblotting Parasite-infected erythrocytes were magnet purified on CS columns (Miltenyi Biotech) and solubilized in 4 × Laemmli buffer as whole infected cells, or permeabilized in 100 units per ml tetanolysin (Sigma) or 0.15% saponin containing 1 × Complete protease inhibitors as described previously 9 , before pellet and supernatant fractions were resuspended in 4 × Laemmli buffer