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Henderson–Hasselbalch equation and pK a The H-H equation describes the titration of an amino acid and can be used to predict the net charge and isoelectric point of a protein The general dissociation of a weak acid, such as a carboxylic acid, is given by the equation: (1) where HA is the protonated form (conjugate acid or associated form) and A − is the unprotonated form (conjugate base, or dissociated form)